שאלה | A CLSM analysis is based on photons that are originated only from the fluorescent focal planes. How can we improve confocality given the fact that the sample is strongly fluorescent and we do not have sample sensitivity issues?

A CLSM analysis is based on photons that are originated only from the fluorescent focal planes. How can we improve confocality given the fact that the sample is strongly fluorescent and we do not have sample sensitivity issues?

		We increase the detector’s pinhole aperture and accordingly adjust the focal planes’ thickness.
		We change the objective to one with a higher magnification.
		We decrease the detector’s pinhole aperture while ensuring that we do not overlook fluorescent information by adjusting the focal planes’ thickness
		We decrease the light source’s pinhole aperture.