שאלה | 31) Explain the principle behind using cell-impermeable dyes to distinguish between live and dead cells in flow cytometry.

31) Explain the principle behind using cell-impermeable dyes to distinguish between live and dead cells in flow cytometry.

		The dye binds specifically to surface markers present only on live cells
		Live cells actively transport the dye into their cytoplasm, resulting in higher fluorescence
		The dye inhibits metabolic activity in live cells, causing them to appear different from dead cells
		Dead cells have damaged cell membranes, allowing the dye to enter and bind intracellular components, while live cells exclude the dye