שאלה | An experiment is set up using two fluorophores: BV421 (emission spectra shown in green) and BV650 (emission spectra shown in purple), using an instrument that has a single LASER: 405 nm, and two bandwith filters: 420/20 nm and 650/30 nm. Which is the true statement?

An experiment is set up using two fluorophores: BV421 (emission spectra shown in green) and BV650 (emission spectra shown in purple), using an instrument that has a single LASER: 405 nm, and two bandwith filters: 420/20 nm and 650/30 nm. Which is the true statement?

		Spillover of BV421 into the BV650 detector needs compensation.
		No compensation is needed because the emission peaks are far apart.
		Compensation will not be necessary for multi-laser flow cytometers.?
		Spillover of BV650 into the BV421 detector needs compensation.