שאלה | For a revision of your paper, you need to repeat a 2-year-old flow cytometry experiment. You find the original antibody vial and your notes indicate a working concentration of 50 ng/mL. What is the best approach?

For a revision of your paper, you need to repeat a 2-year-old flow cytometry experiment. You find the original antibody vial and your notes indicate a working concentration of 50 ng/mL. What is the best approach?

		Use the exact same antibody and working concentration to match the original experiment.
		Re-titrate the antibody, as fluorochromes and antibody performance can change over time.
		Purchase a new antibody of the same clone and use it directly at 50 ng/mL.

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