שאלה | Mutations in Laminin 5 (LAMA5) gene are known to cause a skin blistering disease in human. To study the mechanism, you decided to generate a new mouse model containing LAMA5 point mutations. Mark the steps needed as part of the process of generating the new mouse strain:

Mutations in Laminin 5 (LAMA5) gene are known to cause a skin blistering disease in human. To study the mechanism, you decided to generate a new mouse model containing LAMA5 point mutations. Mark the steps needed as part of the process of generating the new mouse strain:

		Generate induced pluripotent stem cells (iPSCs) from blood cells of a patientcarrier of LAMA5 mutation, then inject LAMA5-mutated iPSCs into a blastocyststage mouse embryo, derivation of chimera mouse
		Generate iPSCs from skin fibroblasts of a patient carrier of LAMA5 mutation, theninject LAMA5-mutated iPSCs into a blastocyst stage mouse embryo, derivation ofchimera mouse
		Use CRISPR genome editing to mutate LAMA5 gene in wild type mouse embryonicstem cells (ESCs), then inject LAMA5-mutated ESCs into a blastocyst stage mouseembryo, derivation of chimera mouse
		Use CRISPR genome editing to mutate LAMA5 gene in mouse skin specific stemcells, then inject the cells into blastocyst stage mouse embryo, derivation ofchimera mouse