בליגציה דרוש ATP

דרוש ATP בחיות ובקטריופאג'ים מויקיפדיה: In animals and bacteriophage, ATP is used as the energy source for the ligation, while In bacteria, NAD+ is used.[3]

לפי הצ'אט ג'י צריך ATP - During the ligation reaction, the ligase uses the energy stored in ATP to activate the 5'-phosphate group of one nucleic acid fragment, making it more reactive and allowing it to attack the 3'-hydroxyl group of another nucleic acid fragment. This results in the formation of a covalent bond between the two fragments, completing the ligation process.