שאלה | The alanine aminoacyl tRNA synthetase enzyme of E. coli recognizes the alanine tRNA only by an unusual G:U base pair at position 70 in the stem of the tRNA. In the laboratory, you make a copy of the alanine tRNA gene that is identical to the wildtype copy except that the anicodon sequence 5' - IGC -3' is replaced with the sequence 5' - UCA -3'. You then add this mutant tRNA gene back into a wildtype E. coli. As a result,

The alanine aminoacyl tRNA synthetase enzyme of E. coli recognizes the alanine tRNA only by an unusual G:U base pair at position 70 in the stem of the tRNA. In the laboratory, you make a copy of the alanine tRNA gene that is identical to the wildtype copy except that the anicodon sequence 5' - IGC -3' is replaced with the sequence 5' - UCA -3'. You then add this mutant tRNA gene back into a wildtype E. coli. As a result,

		1. nothing will change because the mutant tRNA will not function.
		2. all alanines in newly synthesized proteins will be replaced with serine.
		3. proteins will be truncated randomly at different alanine positions.
		4. some proteins will be longer than ususal.
		5. all proteins will be truncated at the first alanine position.
		6. some alanines in newly synthesized proteins will be replaced with serine.

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