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(USMLoid) The DNA polymerases and RNA polymerases of both prokaryotes and eukaryotes make copies of nucleotides sequences - but the RNA polymerases are more prone to error. This doesn't matter because

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The alanine aminoacyl tRNA synthetase enzyme of E. coli recognizes the alanine tRNA only by an unusual G:U base pair at position 70 in the stem of the tRNA. In the laboratory, you make a copy of the alanine tRNA gene that is identical to the wildtype copy except that the anicodon sequence 5' - IGC -3' is replaced with the sequence 5' - UCA -3'. You then add this mutant tRNA gene back into a wildtype E. coli. As a result,

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Core Processes:Translation. This is a difficult one, but is actually basic. Just think the process through. Make diagrams - it may help. This is an example of a difficult question. You can expect one two that demand this level of understanding and analytical skill on the final exam. The correct answer is: some proteins will be longer than ususal.
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Complete the following sentence. The covalent bond between a tRNA and its amino acid is catalysed: השלם את המשפט הבא: הקישור הקוולנטי בין tRNA והחומצה האמינית אליה המתאימה, מזורז -

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(USMLoid) You have identified a mutation in a gene transcribed in the liver. At codon 42 of the expected translation product you find 5'-CAT-3' instead of the normal codon 5'-GAT-3'. This same mutation is found associated with type II diabetes in a large kindred. Which of the following best describes the effect of this change on the protein encoded?

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(USMLoid) Which of the following is a result of the degeneracy of the genetic code?

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In prokaryotes EF-Tu is involved in:

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In humans there are 64 codons. 61 of these code for amino acids. There are 20 amino acids. There are 31 tRNAs. How many different amino acyl tRNA synthetases are there?

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Formation of peptide bonds during protein synthesis is catalysed by:

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Which of the following are correct? Because of the differences in the translation machinery between Prokaryotes and Eukaryotes:

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You have correctly selected 1. The correct answers are: prokaryotic mRNAs are often polycistronic., some drugs are able to stop translation in bacteria, but not in humans.
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The following piece of DNA codes for an mRNA. The top strand is the template strand. The initiation and terminations codons are in the sequence. 5'-AATTACCATGAAATTGTCATCTACTTGTTGATTA-3' 3'-TTAATGGTACTTTAACAGTAGATGAACAACTAAT-5 The sequence of the protein encoded by this transcript is:

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