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From the van Deemter Equation, what does not affect theoretical plate height HTP

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שאלה #56765

done		Material concentration
		Mobile phase flow rate
		Diffusion kinetics
		Material size

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?What is false about silver staining for 2D electrophoresis

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שאלה #56767

		It is 100 times more sensitive than Coomassie Blue staining
		Some stains may show "doughnuts" with missing color in the middle
done		You can’t use glass tools because it emits Ca ions
		Requires high quality water

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The classic method of measuring dietary fibers includes incubation in 95˚C, a disadvantage in this choice is

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שאלה #56768

		Insoluble dietary fibers become soluble
done		Resistant starch breaks down and is not included in final weight
		Digestive enzymes stop working due to denaturation
		It demands a long 16hr incubation

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:The secondary antibody in indirect ELISA

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שאלה #56769

done		Used for signal amplification
		Directly binds the antigen
		Not labeled
		All answers are correct

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what characterize SILAC

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שאלה #56770

		Stable isotopes incorporated during cell growth
		Samples can be mixed after cell harvest to minimize variability
		Is a method that is used to quantify proteins in MS
done		All of the above

נערך לפני 3 שנים Tal Bar

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Which of the following methods removes post translational modifications during fragmentation

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שאלה #56771

		ETD
		ECD
done		CID
		a+b
		a+c

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Signal read out of SPR, MST and ITC is based (respectively) on

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שאלה #56772

		fluorescence intensity, temperature, refractive index
done		refractive index, fluorescence intensity, temperature
		fluorescence intensity, refractive index, temperature
		refractive index, temperature, fluorescence intensity

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What is the purpose of a reflector?

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שאלה #56773

		ionization of a samples in multiple charges
done		. diminishes the spread of flight time of the ions caused by spread in the kinetic energy
		use as matrix in MALDI
		a detector in MS/MS system

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Electron impact ionization method-

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שאלה #56774

done		deposits lots of energy into the molecule causing extensive fragmentation
		relatively gentle ionization method
		electron gets captured by the molecule
		liquid phase samples only

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In Spike protein, what is the role of region S1

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שאלה #56775

		Fusion
		Preservation of alpha helix structure
		Penetration
done		Receptor binding

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