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A280 method used to determine protein concentration. Which amino acid increases the sensitivity of the method

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שאלה #56776

		Serine
done		Tryptophan
		Lysine
		Proline

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Which protein source is suitable for grinding-type extraction method

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שאלה #56777

		Bacteria
		Liver tissue
done		Muscle
		Intracellular proteins

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Which of the following methods is used for eliminating chaperone contamination

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שאלה #56778

		Using ATP-MgCl2 washes
		Multistep purification using different columns (SEC, IEX etc)
		Using ATP-Agarose column for chaperone purification
done		All answers are correct

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Why is there a difficulty in analyzing phosphopeptides

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שאלה #56779

		MS is usually done in a positively charged field, while phosphopeptides are negatively charged.
		phosphopeptides tend to bind to hydrophobic columns.
		phosphopeptides picks are weak due to ionic depletion
done		a+c

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What is incorrect regarding Bradford method

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שאלה #56780

		The Coomassie® dye binds primarily with basic and aromatic side chains
		Coomassie® Brilliant Blue G-250 causes an absorbance at 595 nm.
		The strongest interaction of the Coomassie® dye is with Arginine
done		Only one molecule of dye binds per positive charge on the protein.

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Which of the following is an integer number

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שאלה #56781

		Average mass
		Most abundant mass
done		Nominal mass
		Monoisotopic mass

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A classical lysis buffer for protein solubilization is

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שאלה #56782

done		Urea, nonionic or zwitterionic detergent, DTT, ampholytes
		Urea, thiourea, zwitterionic detergent, DTT or TBP, ampholytes
		SDS, urea, Triton X-100
		Thiol, BME, urea, Tris

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Which is not true about the coronavirus E Protein

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שאלה #56783

		It is a small integral membrane protein
		It is well conserved within each of the different groups of coronaviruses
sentiment_very_satisfied		It is not a structural protein
		It has a net neutral charge

נערך לפני שנה Tal Bar

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Which of the following main component of mass spectroscopy deal with resolving the ions into their characteristics mass components according to their mass-to-charge ratio

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שאלה #56784

		Ion Source
done		Analyzer
		Detector System
		Collision cell

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Which method is not used to quantify protein

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שאלה #56785

		Bradford
done		Gel electrophoresis
		(Bicinchoninic acid assay (BCA
		(UV Absorbance (280 nm

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