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What characterize PFGE (Pulse Field Gel Electrophoresis) method

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שאלה #56796

		Temperature gradient
		Differences between DNA sequences
		Denaturant reagent gradient
done		Large DNA fragments and 3 electric fields directions

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What kind of quantification can be done with iTRAQ (isobaric tag reagent) labeling method

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שאלה #56797

		Relative only
		Absolute only
done		Relative and absolute
		Neither of them

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Chemical matrix is usually used In MALDI method. Which structure do the matrix' molecules have

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שאלה #56798

		Linear molecules with different modifications
done		Ring-shape" molecules, usually with double bonds"
		Linear molecules saturated with double bonds
		Branched molecules

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Which of the following ELISAs doesn't use two different antibodies

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שאלה #56999

done		Direct
		Sandwich
		Competitive
		None of the above

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Which method is recommended for protein relative quantitation in SDS-PAGE?

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שאלה #57000

		Coomassie blue
done		fluorophores
		Silver staining
		radioactive

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Which method can test the spike protein folding of SARS-COV-2?

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שאלה #57001

done		Electron microscopy
		ELISA
		SDS-PAGE
		Fluorescence

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What is the purpose of a reflector in a mass spectrometer

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שאלה #57002

		Ionization of a samples in multiple charges
done		Diminish the spread of flight time of the ions caused by spread in the kinetic energy
		Used as matrix in MALDI
		Is a detector in MS/MS system

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The electron impact ionization method

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שאלה #57003

done		Deposits lots of energy into the molecule causing extensive fragmentation
		Is a relatively gentle ionization method
		Electrons get captured by the molecule
		Is for liquid phase samples only

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In dietary fiber analysis, HPLC is used to

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שאלה #57004

done		Separate non-digestible oligosaccharides from monosaccharides
		Separate soluble and non-soluble fibers
		Determine total protein weight in the food sample
		Determine total fiber weight in the food sample

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Corona virus E protein identification using LC-MS/MS does not require:

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שאלה #57005

		Digestion with Trypsin
		Separation of peptides by LC.
		Fragmentation of peptide ions using MS/MS
done		The polarization of the magnetic nuclear spins by a weak oscillating magnetic field

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