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In a FITC single-stained sample, why can an apparent PE signal be observed?
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An experiment is set up using two fluorophores: BV421 (emission spectra shown in green) and BV650 (emission spectra shown in purple), using an instrument that has a single LASER: 405 nm, and two bandwith filters: 420/20 nm and 650/30 nm. Which is the true statement?
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Which control sample is essential for manual compensation in this experiment?
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To manually calculate compensation in this experiment, which parameter is necessary?
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Why is it important to include live/dead cell discrimination in every experiment of flow cytometry?
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What principle underlies dye exclusion viability assays such as PI or 7-AAD?
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Why can PI and 7-AAD not be used to assess viability in fixed cells?
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What staining pattern do you expect to see in cells at early apoptosis?
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